Influenza A virus hemagglutinin is a B cell-superstimulatory lectin
Identifieur interne : 001C67 ( Main/Exploration ); précédent : 001C66; suivant : 001C68Influenza A virus hemagglutinin is a B cell-superstimulatory lectin
Auteurs : Ortwin Rott [France] ; Jeannine Charreire [France] ; Evelyne Cash [France]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 1996-02-01.
English descriptors
- KwdEn :
- Teeft :
- Aggregation, Amino acids, Anders, Antigenic site, Binding site, Cell activation, Cell aggregates, Cell aggregation, Cell mitogenicity, Cell proliferation, Cell stimulation, Cell surface marker expression, Chimeric, Control cultures, Culture medium, Culture period, Culture plates, Different viruses, Direct interaction, Fusion peptide, Generous gift, Globular region, Glycoprotein, Hemagglutinin, High degree, Homotypic aggregation, Hypervariable loop region, Immunol, Influenza, Influenza virus, Influenza virus hemagglutinin, Influenza virus transfectants, Influenza viruses, Inhibitor, Inhibitors tunicamycin, Light microscopy, Loop region, Lymphocyte, Lymphocyte activation, Lymphocyte receptors, Microtiter plates, Mitogenic activity, Molecular basis, Mutant viruses, Neuraminidase treatment, Other experiments, Palese, Proliferative, Proliferative responses, Reassortant viruses, Receptor, Receptor binding, Receptor determinants, Responder, Responder cells, Rott, Simultaneous addition, Sinai school, Subtypes, Terminal sialic acid residues, Triplicate determinations, Vast differences, Viral, Viral envelope protein, Virus, Virus infection, Wild type.
Abstract
Abstract: Influenza A viruses display T cell-independent polyclonal B cell-activating properties which are mediated by the B cell-superstimulatory envelope glycoprotein hemagglutinin (HA). In this report, the receptor-binding requirements for B cell activation by influenza viruses were expected. Neuraminidase treatment of resting mature B cells from BALB/c mice abrogated late (proliferation/immunoglobulin synthesis), early (up-regulation of cell surface markers, including CD25, B220, and B7-1) and veryearly events (homotypic adhesion) in virus-responding B lymphocytes. Similarly, pretreatment of murine responder cells with different inhibitors ofN-glycosylation (tunicamycin, deoxymannojirimycin) significantly suppressed subsequent B lymphocyte activation by HA, but not control responses to lipopolysaccharide or anti-μ. Assays with chimeric HA transfectants, expressing the loop region of epitope B (amino acids 155–160) of the globular head of H2 (high B cell-stimulatory subtype) or H3 (medium-stimulatory subtype) on the protein backbone of a low-stimulatory subtype (H1) failed to alter the B cell-stimulatory activity of the virus, suggesting that the hypervariable loop region is not crucial in determining the B cell-activating properties of the protein. Collectively, our results imply that the B cell-superstimulatory function of influenza virus HA is not mediated by a direct protein/protein interaction, but via binding of HA to terminal sialic acid residues on cell surface receptor glycoproteins. These findings identify the influenza virus HA glycoprotein as the first viral lectin with lymphocyte-activating properties.
Url:
DOI: 10.1007/BF02456134
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001218
- to stream Istex, to step Curation: 001218
- to stream Istex, to step Checkpoint: 000A46
- to stream Main, to step Merge: 001D35
- to stream Main, to step Curation: 001C67
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influenza A virus hemagglutinin is a B cell-superstimulatory lectin</title><author><name sortKey="Rott, Ortwin" sort="Rott, Ortwin" uniqKey="Rott O" first="Ortwin" last="Rott">Ortwin Rott</name></author><author><name sortKey="Charreire, Jeannine" sort="Charreire, Jeannine" uniqKey="Charreire J" first="Jeannine" last="Charreire">Jeannine Charreire</name></author><author><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D7310E89F1F62F7DF84F75B4B166EAA695634DE0</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1007/BF02456134</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-RMF521W1-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001218</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001218</idno><idno type="wicri:Area/Istex/Curation">001218</idno><idno type="wicri:Area/Istex/Checkpoint">000A46</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A46</idno><idno type="wicri:doubleKey">0300-8584:1996:Rott O:influenza:a:virus</idno><idno type="wicri:Area/Main/Merge">001D35</idno><idno type="wicri:Area/Main/Curation">001C67</idno><idno type="wicri:Area/Main/Exploration">001C67</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Influenza A virus hemagglutinin is a B cell-superstimulatory lectin</title><author><name sortKey="Rott, Ortwin" sort="Rott, Ortwin" uniqKey="Rott O" first="Ortwin" last="Rott">Ortwin Rott</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U. 283, Hôpital Cochin, Université René Descartes, 27 rue du Fg. St.-Jacques, F-75674, Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Charreire, Jeannine" sort="Charreire, Jeannine" uniqKey="Charreire J" first="Jeannine" last="Charreire">Jeannine Charreire</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U. 283, Hôpital Cochin, Université René Descartes, 27 rue du Fg. St.-Jacques, F-75674, Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U. 283, Hôpital Cochin, Université René Descartes, 27 rue du Fg. St.-Jacques, F-75674, Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="ISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1996-02-01">1996-02-01</date><biblScope unit="volume">184</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="193">193</biblScope></imprint><idno type="ISSN">0300-8584</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8584</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>B cell activation</term><term>Influenza virus</term><term>Superantigen</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Aggregation</term><term>Amino acids</term><term>Anders</term><term>Antigenic site</term><term>Binding site</term><term>Cell activation</term><term>Cell aggregates</term><term>Cell aggregation</term><term>Cell mitogenicity</term><term>Cell proliferation</term><term>Cell stimulation</term><term>Cell surface marker expression</term><term>Chimeric</term><term>Control cultures</term><term>Culture medium</term><term>Culture period</term><term>Culture plates</term><term>Different viruses</term><term>Direct interaction</term><term>Fusion peptide</term><term>Generous gift</term><term>Globular region</term><term>Glycoprotein</term><term>Hemagglutinin</term><term>High degree</term><term>Homotypic aggregation</term><term>Hypervariable loop region</term><term>Immunol</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus hemagglutinin</term><term>Influenza virus transfectants</term><term>Influenza viruses</term><term>Inhibitor</term><term>Inhibitors tunicamycin</term><term>Light microscopy</term><term>Loop region</term><term>Lymphocyte</term><term>Lymphocyte activation</term><term>Lymphocyte receptors</term><term>Microtiter plates</term><term>Mitogenic activity</term><term>Molecular basis</term><term>Mutant viruses</term><term>Neuraminidase treatment</term><term>Other experiments</term><term>Palese</term><term>Proliferative</term><term>Proliferative responses</term><term>Reassortant viruses</term><term>Receptor</term><term>Receptor binding</term><term>Receptor determinants</term><term>Responder</term><term>Responder cells</term><term>Rott</term><term>Simultaneous addition</term><term>Sinai school</term><term>Subtypes</term><term>Terminal sialic acid residues</term><term>Triplicate determinations</term><term>Vast differences</term><term>Viral</term><term>Viral envelope protein</term><term>Virus</term><term>Virus infection</term><term>Wild type</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Influenza A viruses display T cell-independent polyclonal B cell-activating properties which are mediated by the B cell-superstimulatory envelope glycoprotein hemagglutinin (HA). In this report, the receptor-binding requirements for B cell activation by influenza viruses were expected. Neuraminidase treatment of resting mature B cells from BALB/c mice abrogated late (proliferation/immunoglobulin synthesis), early (up-regulation of cell surface markers, including CD25, B220, and B7-1) and veryearly events (homotypic adhesion) in virus-responding B lymphocytes. Similarly, pretreatment of murine responder cells with different inhibitors ofN-glycosylation (tunicamycin, deoxymannojirimycin) significantly suppressed subsequent B lymphocyte activation by HA, but not control responses to lipopolysaccharide or anti-μ. Assays with chimeric HA transfectants, expressing the loop region of epitope B (amino acids 155–160) of the globular head of H2 (high B cell-stimulatory subtype) or H3 (medium-stimulatory subtype) on the protein backbone of a low-stimulatory subtype (H1) failed to alter the B cell-stimulatory activity of the virus, suggesting that the hypervariable loop region is not crucial in determining the B cell-activating properties of the protein. Collectively, our results imply that the B cell-superstimulatory function of influenza virus HA is not mediated by a direct protein/protein interaction, but via binding of HA to terminal sialic acid residues on cell surface receptor glycoproteins. These findings identify the influenza virus HA glycoprotein as the first viral lectin with lymphocyte-activating properties.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Rott, Ortwin" sort="Rott, Ortwin" uniqKey="Rott O" first="Ortwin" last="Rott">Ortwin Rott</name></region><name sortKey="Cash, Evelyne" sort="Cash, Evelyne" uniqKey="Cash E" first="Evelyne" last="Cash">Evelyne Cash</name><name sortKey="Charreire, Jeannine" sort="Charreire, Jeannine" uniqKey="Charreire J" first="Jeannine" last="Charreire">Jeannine Charreire</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C67 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C67 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:D7310E89F1F62F7DF84F75B4B166EAA695634DE0
|texte= Influenza A virus hemagglutinin is a B cell-superstimulatory lectin
}}
| This area was generated with Dilib version V0.6.33. |  |